Acceptable Samples

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Human Sample Volume Requirements
MAP Volume Required
Serum or plasma Other fluids*
Human DiscoveryMAP® 1 mL 3 mL
Human ExplorerMAP™ 400 µL 800 µL
Human OncologyMAP® 500 µL 2 mL
HumanMAP® 120 µL 650 µL
Human CardiovascularMAP® 350 µL 700 µL
Human AngiogenesisMAP® 200 µL 500 µL
Human InflammationMAP® 100 µL 200 µL
Human ImmunoMAP® 120 µL 650 µL
Human NeuroMAP™ 150 µL 300 µL
Human MetabolicMAP® 100 µL 350 µL
Human KidneyMAP® 100 µL 350 µL
Human TruCulture® MAP 100 µL 200 µL (TruCulture® Supernatant)
Human CytokineMAP® A 50 µL 100 µL
Human CytokineMAP® B 50 µL 100 µL
Vectra® DA** 300 µL n/a
*Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.
**Serum is the only accepted sample type for Vectra DA testing. If MAP and Vectra DA testing services are requested, please provide a separate electronic sample manifest and sample aliquot for Vectra DA testing. All Vectra DA testing is performed at Crescendo Biosciences, Inc.
Simoa™ Services
Biomarker LLOQ*
(Serum and Plasma)
LLOQ*
(Undiluted Samples)
Volume Required
Serum or plasma Other fluids**
Interferon gamma (IFN-gamma) 0.019 pg/mL 0.0095 pg/mL 160 µL 300 µL
Interleukin-1 beta (IL-1 beta) 0.0313 pg/mL 0.016 pg/mL 160 µL 300 µL
Interleukin-6 (IL-6) 0.078 pg/mL 0.0039 pg/mL 20 µL 300 µL
Interleukin-10 (IL-10) 0.0368 pg/mL 0.0092 pg/mL 80 µL 300 µL
Tumor Necrosis Factor-alpha (TNF-α) 0.091 pg/mL 0.023 pg/mL 80 µL 300 µL

*Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.

**Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.

Rodent Sample Volume Requirements
MAP Volume Required
Serum or plasma Other fluids*
RodentMAP® 70 µL 300 µL
Mouse InflammationMAP® 50 µL 150 µL
BioPlex Pro™ RBM Rat Kidney Toxicity Service NA 200 µL urine

*Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.
If your volumes are not sufficient, please contact Myriad RBM for other options and information.


  • Filter paper blood spots
  • Other fluid samples including:
    • Tissue culture supernatant – Caution- biotin concentrations must be less than 0.65µM (<160 ng/mL)
    • Urine
    • Cerebrospinal Fluid
    • Peritoneal fluid
    • Cell lysates
    • BALF (broncheoalveolar lavage fluid)
    • Tissue homogenates – Procedure
    • Synovial fluid
  • Do not send whole blood
  • Contact a Myriad RBM Representative to confirm sample volume requirements
  • If your volumes are not sufficient, please contact Myriad RBM for other options and information.